C. elegans Transgenics
Knockout
Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity. Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE. Nat Commun. 2017 Jun 6;8:15558.
MosSCI
Developmental dynamics of gene expression and alternative polyadenylation in the Caenorhabditis elegans germline. West SM, Mecenas D, Gutwein M, Aristizábal-Corrales D, Piano F, Gunsalus KC. Genome Biol. 2018 Jan 24;19(1):8.
Linking Genomic and Metabolomic Natural Variation Uncovers Nematode Pheromone Biosynthesis. Falcke JM, Bose N, Artyukhin AB, Rödelsperger C, Markov GV, Yim JJ, Grimm D, Claassen MH, Panda O, Baccile JA, Zhang YK, Le HH, Jolic D, Schroeder FC, Sommer RJ. Cell Chem Biol. 2018 Jun 21;25(6):787-796.e12.
Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity. Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE. Nat Commun. 2017 Jun 6;8:15558.
Mixtures of chemical pollutants at European legislation safety concentrations: how safe are they? Carvalho, RN; Arukwe, A; Ait-Aissa, S; Bado-Nilles, A; Balzamo, S; Baun, A; Belkin, S; Blaha, L; Brion, F; Conti, D; Creusot, N; Essig, Y; Ferrero, VE; Flander-Putrle, V; Fürhacker, M; Grillari-Voglauer, R; Hogstrand, C; Jonáš, A; Kharlyngdoh, JB; Loos, R; Lundebye, AK; Modig, C; Olsson, PE; Pillai, S; Polak, N; Potalivo, M; Sanchez, W; Schifferli, A; Schirmer, K; Sforzini, S; Stürzenbaum, SR; Søfteland, L; Turk, V; Viarengo, A; Werner, I; Yagur-Kroll, S; Zounková, R; Lettieri, T. Toxicol Sci. 2014 Sep;141(1):218-33.
Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis. Ding, WY; Ong, HT; Hara, Y; Wongsantichon, J; Toyama, Y; Robinson, RC; Nédélec, F; Zaidel-Bar, R. J Cell Biol. 2017 May 1;216(5):1371-1386.
The secreted MSP domain of C. elegans VAPB homolog VPR-1 patterns the adult striated muscle mitochondrial reticulum via SMN-1. Schultz, J; Lee, SJ; Cole, T; Hoang, HD; Vibbert, J; Cottee, PA; Miller, MA; Han, SM. Development. 2017 Jun 15;144(12):2175-2186.
MED GATA factors promote robust development of the C. elegans endoderm. Maduro, MF; Broitman-Maduro, G; Choi, H; Carranza, F; Wu, AC; Rifkin, SA. Dev Biol. 2015 Aug 1;404(1):66-79.
The 14-3-3 protein PAR-5 regulates the asymmetric localization of the LET-99 spindle positioning protein. Wu, JC; Espiritu, EB; Rose, LS. Dev Biol. 2016 Apr 15;412(2):288-297.
Expression of human Bcl-xL (Ser49) and (Ser62) mutants in Caenorhabditis elegans causes germline defects and aneuploidy. Baruah, PS; Beauchemin, M; Parker, JA; Bertrand, R. PLoS One. 2017 May 8;12(5):e0177413.
Longevity Genes Revealed by Integrative Analysis of Isoform-Specific daf-16/FoxO Mutants of Caenorhabditis elegans. Chen, AT; Guo, C;Itani, OA; Budaitis, BG; Williams, TW; Hopkins, CE; McEachin, RC; Pande, M;Grant, AR; Yoshina, S; Mitani, S; Hu, PJ. Genetics. 2015 Oct;201(2):613-29.
Matefin/SUN-1 Phosphorylation on Serine 43 Is Mediated by CDK-1 and Required for Its Localization to Centrosomes and Normal Mitosis in C. elegans Embryos. Zuela, N; Gruenbaum, Y. Cells. 2016 Feb 24;5(1).
Point Mutation
Regulation of the sperm-to-oocyte transition in Caenorhabditis briggsae hermaphrodites by the Cbr-met-2 and Cbr-fem-3 genes. Berenson AL, Baird SE. Mol Reprod Dev. 2018 Jun;85(6):532-542.
NALCN channelopathies: Distinguishing gain-of-function and loss-of-function mutations. Bend, EG; Si, Y; Stevenson, DA; Bayrak-Toydemir, P; Newcomb, TM; Jorgensen, EM; Swoboda, KJ. Neurology. 2016 Sep 13;87(11):1131-9.
An RNAi-based suppressor screen identifies interactors of the Myt1 ortholog of Caenorhabditis elegans. Allen, AK; Nesmith, JE; Golden, A. G3 (Bethesda). 2014 Oct 8;4(12):2329-43.
LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during cytokinesis.HONG, Y; Sonneville, R; Wang, B; Scheidt, V; Meier, B; Woglar, A; Demetriou, S; Labib, K; Jantsch, V; Gartner, A. Nat Commun. 2018 Feb 20;9(1):728.
Precise Deletion
A Role in Apoptosis Regulation for the rad-51 Gene of Caenorhabditis elegans. Germoglio M, Adamo A. Genetics. 2018 Aug;209(4):1017-1028.
WAH-1/AIF regulates mitochondrial oxidative phosphorylation in the nematode Caenorhabditis elegans. Troulinaki K, Büttner S, Marsal Cots A, Maida S, Meyer K, Bertan F, Gioran A, Piazzesi A, Fornarelli A, Nicotera P, Bano D. Cell Death Discov. 2018 Jan 29;4:2.
Tagging
LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis. Hong Y, Sonneville R, Wang B, Scheidt V, Meier B, Woglar A, Demetriou S, Labib K, Jantsch V, Gartner A. Nat Commun. 2018 Feb 20;9(1):728.
The myosin light chain kinase, MLCK-1 relocalizes during C. elegans ovulation to promote actomyosin bundle assembly and drive contraction. Kelley CA, Wirshing ACE, Zaidel-Bar R, Cram EJ. Mol Biol Cell. 2018 Aug 8;29(16):1975-1991.
CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis. Sonneville, R; Moreno, SP; Knebel, A; Johnson, C; Hastie, CJ; Gartner, A; Gambus, A; Labib, K. Nat Cell Biol. 2017 May;19(5):468-479.
Olfactory stem cells reveal MOCOS as a new player in autism spectrum disorders. Féron, F; Gepner, B; Lacassagne, E; Stephan, D; Mesnage, B; Blanchard, MP; Boulanger, N; Tardif, C; Devèze, A; Rousseau, S; Suzuki, K; Izpisua Belmonte, JC; Khrestchatisky, M; Nivet, E; Erard-Garcia, M. Mol Psychiatry. 2016 Sep;21(9):1215-24.
Ribonuclease-Mediated Control of Body Fat. Habacher, C; Guo, Y; Venz, R; Kumari, P; Neagu, A; Gaidatzis, D; Harvald, EB; Færgeman, NJ; Gut, H; Ciosk, R. Dev Cell. 2016 Nov 7;39(3):359-369.
Replication-Independent Histone Variant H3.3 Controls Animal Lifespan through the Regulation of Pro-longevity Transcriptional Programs. Piazzesi, A; Papić, D; Bertan, F; Salomoni, P; Nicotera, P; Bano, D. Cell Rep. 2016 Oct 18;17(4):987-996.
The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development. Cottee, PA; Cole, T; Schultz, J; Hoang, HD; Vibbert, J; Han, SM; Miller, MA. Development. 2017 Jun 15;144(12):2187-2199.
Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans. Smolentseva, O; Gusarov, I; Gautier, L; Shamovsky, I; DeFrancesco, AS; Losick, R; Nudler, E. Sci Rep. 2017 Aug 2;7(1):7137.
Regulation of DJ-1 by Glutaredoxin 1 in Vivo: Implications for Parkinson’s Disease. Johnson, WM; Golczak, M; Choe, K; Curran, PL; Miller, OG; Yao, C; Wang, W; Lin, J; Milkovic, NM; Ray, A; Ravindranath, V; Zhu, X; Wilson, MA; Wilson-Delfosse, AL; Chen, SG; Mieyal, JJ. Biochemistry. 2016 Aug 16;55(32):4519-32.
Caenorhabditis elegans BUB-3 and SAN-1/MAD3 Spindle Assembly Checkpoint Components Are Required for Genome Stability in Response to Treatment with Ionizing Radiation. Bertolini, S;Wang, B;Meier, B;Hong, Y;Gartner, A. G3 (Bethesda). 2017 Dec 4;7(12):3875-3885.
A Bystander Mechanism Explains the Specific Phenotype of a Broadly Expressed Misfolded Protein. Klabonski, L;Zha, J;Senthilkumar, L;Gidalevitz, T. PLoS Genet. 2016 Dec 7;12(12):e1006450.
A Differentiation Transcription Factor Establishes Muscle-Specific Proteostasis in Caenorhabditis elegans. Bar-Lavan, Y; Shemesh, N; Dror, S; Ofir, R; Yeger-Lotem, E; Ben-Zvi, A. PLoS Genet. 2016 Dec 30;12(12):e1006531.
The nphp-2 and arl-13 genetic modules interact to regulate ciliogenesis and ciliary microtubule patterning in C. elegans. Warburton-Pitt, SR; Silva, M; Nguyen, KC; Hall, DH; Barr, MM. PLoS Genet. 2014 Dec 11;10(12):e1004866.
Zebrafish Transgenics
Knockout
Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Yu, M; Liu, Y; Li, J; Natale, BN; Cao, S; Wang, D; Amack, JD; Hu, H. Biol Open. 2016 Nov 15;5(11):1662-1673.
wMicroTracker
Screening of a drug repurposing library with a nematode motility assay identifies promising anthelmintic hits against Cooperia oncophora and other ruminant parasites. Liu M., Landuyt B., Klaassen H., Geldhof P., Luyten W. Veterinary Parasitology. 2018 Dec.
C. elegans Development and Activity Test Detects Mammalian Developmental Neurotoxins. Piper Reid Hunt; Nicholas Olejnik; Keenan D. Bailey; Cory A. Vaught; Robert L. Sprando. 2018 Nov; 121:583-592
Active principles of Tetradenia riparia. IV. Anthelmintic activity of 8(14),15-sandaracopimaradiene-7α,18-diol. Luc Van Puyvelde; Maoxuan Liu; Cedrick Veryser; Wim M. De Borggraeve; Joseph Mungarulire; Marie Jeanne Mukazayire; Walter Luyten. 2018 Apr; 216:229-232
Rescue of ATXN3 neuronal toxicity in C . elegans by chemical modification of ER stress. Fardghassemi Y ; Tauffenberger A ; Gosselin S ; Parker JA. Dis Model Mech. 2017 Dec 19;10(12):1465-1480.
A rapid chemical genetic screen utilizing impaired movement phenotypes in C . elegans : Input into genetics of neurodevelopmental disorders. Schmeisser K ; Fardghassemi Y; Parker JA. Exp Neurol. 2017 Jul;293:101-114.
Twitchin kinase inhibits muscle activity. Matsunaga Y ; Hwang H ; Franke B ; Williams R ; Penley M ; Qadota H ; Yi H ; Morran LT ; Lu H ; Mayans O ; Benian GM. Mol Biol Cell. 2017 Jun 15;28(12):1591-1600.
Neurodegeneration in C. elegans models of ALS requires TIR-1/Sarm1 immune pathway activation in neurons. Veriepe J ; Fossouo L ; Parker JA. Nat Commun. 2015 Jun 10;6:7319.
Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle. Matsunaga Y ; Qadota H ; Furukawa M ; Choe HH ; Benian GM. Mol Biol Cell. 2015 Jun 1;26(11):2096-111.
Insulin/IGF-1 receptor signaling enhances biosynthetic activity and fat mobilization in the initial phase of starvation in adult male C. elegans. Tan KT ; Luo SC ; Ho WZ ; Lee YH. Cell Metab. 2011 Sep 7;14(3):390-402.
ScreenChip System
The transcription factor NHR-8: A new target to increase ivermectin efficacy in nematodes. C. Ménez, M. Alberich, E. Courtot, F. Guegnard, A. Blanchard, H. Aguilaniu, A. Lespine. https://doi.org/10.1371/ journal.ppat.1007598
Electrophysiological measures of aging pharynx function in C. elegans reveal enhanced organ functionality in older, long-lived mutants. J. Russell, N. Burnaevskiy, B. Ma, M. Mailig, F. Faust, M. Crane, M. Kaeberlein, A. Mendenhall. The Journals of Gerontology: Series A, Nov. 2017.
Tool-Driven Advances in Neuropeptide Research from a Nematode Parasite Perspective. CJ McCoy, LE Atkinson, E Robb, NJ Marks, AG Maule, A Mousley. Trends Parasitol. 2017 Dec;33(12):986-1002. doi: 10.1016/j.pt.2017.08.009. Epub 2017 Oct 3.
Rabies virus modifies host behaviour through a snake-toxin like region of its glycoprotein that inhibits neurotransmitter receptors in the CNS. K. Hueffer, S. Khatri, S. Rideout, M. Harris, R. Papke, C. Stokes & M. Schulte. Nature Scientific Reports 7, Article number: 12818 (2017)
Distinct unfolded protein responses mitigate or mediate effects of nonlethal deprivation of C. elegans sleep in different tissues. J. Sanders, M. Scholz, I, Merutka and D. Biron. BMC Biology 2017 15:67.
Precision deletion of the entire coding sequence of the mod-5 locus causes increase in pharyngeal pumping frequency. T. Brock, S. Pop, C. Bradford, J. Lawson, L. Resch, C. Hopkins. Micropublication:biology. June 2017.
Basal pharyngeal pumping elevated in C. elegans mod-5 mutants. T. Hiebert, A. Chicas-Cruz, and K. McCormick. Micropublication:biology. June 2017.
Reduced pharyngeal pumping rates observed in tph-1 mutants using microfluidic electropharyngeogram (EPG) recordings. T. Hiebert, A. Chicas-Cruz, and K. McCormick. Micropublication:biology. Feb. 2017.
Mutations in KCNQ potassium channels cause pharyngeal pumping defects in C. elegans. Y. Clovis, A. Webb, C. Turner, and W. Roberts. Micropublication:biology. Nov. 2016.
Trehalose Extends Healthspan in C. elegans. K. Robinson and K. McCormick. Micropublication:biology. Oct. 2016.
CherryTemp
Using fast-acting temperature-sensitive mutants to study cell division in Caenorhabditis elegans.
T Davies, S Sundaramoorthy, SN Jordan, M Shirasu-Hiza, J Dumont, JC Canman. Methods Cell Biol. 2017 June;137:283-306
Inhibition of ectopic microtubule assembly by the kinesin-13 KLP-7 prevents chromosome segregation and cytokinesis defects in oocytes. E Gigant, M Stefanutti, K Laband, A Gluszek-Kustusz, F Edwards, B lacroix, G Maton, JC Canman, JPI Welburn, J Dumont. Development. 2017 May 1;144(9):1674-1686.
Chromosome segregation occurs by microtubule pushing in oocytes. K Laband, R Le Borgne, F Edwards, M Stefanutti, JC Canman, JM Verbavatz, J Dumont. Nat Commun. 2017 Nov 14;8(1):1499.
FLIRT: fast local infrared thermogenetics for subcellular control of protein function. SM Hirsch, S Sundaramoorthy, T Davies, Y Zhuravley, JC Waters, M Shirasu-Hiza, J Dumont, JC Canman. Nat Methods. 2018 Nov;15(11):921-923.
Other Microfluidic EPG Platforms
Anthelmintic Drug Actions in Resistant and Susceptible C. elegans Revealed by Electrophysiological Recordings in a Multichannel Microfluidic Device. J. Weeks, K. Robinson, S. Lockery, W. Roberts. IJP: Drugs and Drug Resistance. 2018 Oct 30; 8: 607-628.
Sertraline, Paroxetine, and Chlorpromazine Are Rapidly Acting Anthelmintic Drugs Capable of Clinical Repurposing. J. Weeks, W. Roberts, C. Leasure C, B. Suzuki, K. Robinson, H. Currey, P. Wangchuk, R. Eichenberger, A. Saxton, T. Bird, B. Kraemer, A. Loukas A, J. Hawdon, C. Caffre, N. Liachko. Sci Rep. 2018 Jan 17;8(1):975.
Microfluidic EPG Recordings Show Striking Pharyngeal Pumping Phenotype in a C. elegans Alzheimer’s Disease Model. J. Weeks, K. Robinson, B. Wanjeri, P. Copenhaver, W. Roberts. Micropublication:biology. Oct. 2016.
Microfluidic platform for electrophysiological recordings from host-stage hookworm and Ascaris suum larvae: A new tool for anthelmintic research. J. Weeks, W. Roberts, K. Robinson, M. Keaney, J. Vermeire, J. Urban, Jr., S. Lockery and J. Hawdon. Int J Parasitol Drugs Drug Resist. 2016 Dec; 6(3): 314–328. doi: 10.1016/j.ijpddr.2016.08.001.
A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans. S. Lockery, S. Hulme, W. Roberts, K. Robinson, A. Laromaine, T. Lindsay, G. Whitesides, and J. Weeks. Lab Chip. 2012 Jun 21; 12(12): 2211–2220. doi: 10.1039/c2lc00001f.
