I assessed the risk of contamination of the freeze dried bacteria on a plate and made preliminary observations about N2 development on this food source. It seems that when cultivated on freeze dried bacteria lawns, worms seem to avoid the lawns and show developmental delay or at least a larger size discrepancy between individuals that under normal conditions.
Great article that highlights some of the strengths of C. elegansfor investigating higher level processes beyond what can be easily studied using cell culture. In this case alternative pre-mRNA splicing. Also helped expose me to the a new descriptor for C. elegans worms – intron-rich.
There are approximately 7,000 rare diseases in humans, ~80% of which are monogenic. A rare disease is defined as affecting less than one in 1,500 people. Combined, these rare diseases affect nearly 1 in 10 Americans (25 to 30 million people), and treatments only exist for around 5% of these diseases. Thanks to the advent of whole genome sequencing, the gene(s) responsible for many rare diseases are known, opening the door for more comprehensive studies.
I started my experiments based on the plan. I tried different levels of concentration. The initial results were mixed.
I have had my eyes on LabTie’s freeze dried bacteria for a couple of months now. It could be a great way to reduce the variability of food source and streamline worm maintenance without having to deal with overnight incubation. I decided to put this food source to the test before potentially making it part of my worms’ diet.
If this food source turns out to be of good quality, it would definitely be a game changer in our lab.
Recent improvements in our knowledge of the natural environment of C. elegans should make us cautious about the conclusions we draw from observations of worm behavior on agar plates. Do microfluidic devices offer a more naturalistic alternative?
Robbie Rae from Liverpool John Moores University discussed his latest research on how he showed that snails use their shells to trap and kill parasites in a fascinating article published on the independent news site The Conversation. His study has shown that snails evolved to use their shells in this battle as a way to encapsulate and kill the parasites as part of their immune system.
Non-mammalian model organisms are typically used in early research to deliver fast answers to a discovery problem. The most popular model organisms in biological and biomedical research are the fruit fly Drosophila melanogaster, the zebrafish and the nematode C. elegans. We provide an overview of the advantages and limitations of these organisms as models for human diseases in this post
Interesting data using C. elegans for studying the impact your gut microbiome may have on your lifespan and healthspan from the Wang Lab at Baylor College of Medicine. The study highlights the power of C. elegans as a tool for whole animal discovery – the work would have been impossible to carry out using cultured cells and would have been prohibitively expensive in mice.
We discovered that in the absence of cholesterol, NPC mutants display a lower pharyngeal pumping frequency and a longer duration between pumps than control N2 animals. This is the progress we made in our collaborative research project with Perlara and investigation into the lysosomal disorder, Niemann-Pick Type C (NPC).