Optimized Plasmids for Expression in C. elegans
Our optimized plasmids enable improved efficiencies in making transgenics. They have been rigorously tested and are routinely used in our custom production of transgenics. Your workflow can be made more efficient with our optimized plasmids. We currently offer 4 plasmids:
- pNU1228: sqt-1 marker plasmid (100ng/ul, 15 ul volume)
- pNU675: myo-2::GFP marker plasmid (15ng/uL, 15uL volume)
- pNU1254: hygR selection plasmid (100ng/ul, 15 ul volume)
- pNU406 “Super-rol” plasmid (rol-6(su1006)) (10ng/uL, 15uL volume)
sqt-1 marker plasmid (pNU1228)
The sqt-1 plasmid encodes for a cuticle collagen expression in C elegans. Composition is sqt-1p::SQT-1(genomic)::sqt-1utr. A single point mutation (e1350) within the gene produces a morphological phenotype similar to Rol due to an improper cuticle formation. Expression of SQT-1 is seen during L2, L3, and L4 larvae and adult stages, but not L1 or dauer larvae. View the Plasmid Map.
myo-2::GFP marker plasmid (pNU675)
The myo-2::GFP construct expresses GFP in the pharynx in C. elegans. Composition is myo-2p::eGFP(3x-syntrons)::tbb-2utr. The myo-2 promoter drives GFP exclusively in pharynx of the animal. View the Plasmid Map
hygR selection plasmid (pNU1254)
The HygR plasmid encodes for hygromycin resistance, a useful selection tool, resulting in broad expression of hygromycin B phosphotransferase (HPT) in C. elegans tissues. The HygR plasmid is commonly added into an injection mix at a final concentration of 10 ng/uL in a 100 ng/uL total DNA mix. Sufficient accumulation of HPT expression occurs 24 hours post injection and survival starting two days post Hygromycin B exposure (0.25 mg/mL) indicates resistance. By day five of post hygromycin exposure, only resistant animals are present. View the Plasmid Map.
“Super-rol” plasmid (pNU406)
The “Super-rol” plasmid is an optimized version of a popular co-injection marker that encodes a mutant collagen that induces a dominant “roller” phenotype. This easy to detect phenotype is compatible with many co-injected transgenes. The “Super-rol” plasmid is commonly added to an injection mix at a final concentration of 40 ng/uL. View the Plasmid Map.
- – USA: $15 flat rate via USPS or use your own shipper account.
- – International: $120 or use your own shipper account.