The short generation time and invariant developmental lineage have made C. elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging.
Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution.
NemaMetrix is a licensed CRISPR solutions provider. We specialize in building these tools to help you accomplish your research goal.
Heat-shock promoters allow you to control gene expression through external temperature changes. Cre-recombinase can be placed behind one of these promoters, for activation to occur only when the organism is subjected to heat.
Introduction of promoters allows you to control when a gene region is expressed. This is appealing for allele floxing in which Cre-recombinase can cause a time-specific or developmental-stage deletion event.