Gene knockouts are a commonly used tool for biologists to understand gene function. Examination of phenotypes after gene deletion can reveal insights into the role of that gene in the organism.
Using CRISPR/Cas9, NemaMetrix can delete the entire coding sequence of a gene. You can then study the phenotype of the true null allele with no concern that any protein function remains.
Our Knockout service can delete up to 36k bp. Any genomic region can be targeted for deletion.
Service Details (price reflects academic pricing)
|Service Package||Price||Est. Delivery Time|
|Full Build||$4,075||6 - 8 Weeks|
|Candidate Lines||$2,554||3 - 4 Weeks|
|Custom Injection Mix||$995||1 - 2 Weeks|
DOT1L complex suppresses transcription from enhancer elements and ectopic RNAi in Caenorhabditis elegans. Esse R, Gushchanskaia ES, Lord A, Grishok, A. RNA. 2019.
DOT1L suppresses nuclear RNAi originating from enhancer elements in Caenorhabditis elegans. Esse R, Gushchanskaia E, Lord A, Grishok A. BioRxiv. 2018. 1-46.
Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity. Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE. Nat Commun. 2017 Jun 6;8:15558.