Gene knockouts are a commonly used tool for biologists to understand gene function. Examination of phenotypes after gene deletion can reveal insights into the role of that gene in the organism.

Using CRISPR/Cas9, NemaMetrix can delete the entire coding sequence of a gene. You can then study the phenotype of the true null allele with no concern that any protein function remains.

Our Knockout service can delete up to 24 kb. Any genomic region can be targeted for deletion.

Show case: mod-5 deletion project

Publication: precise deletion of the mod-5 mutant