C. elegans lysis methods comparison
 
Cat No. PCR101, PCR301

Worm Lysis Kit

$95.00$645.00

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Shipping Details:
- USA: $15 flat rate via USPS or use your own shipper account.
- International: $120 or use your own shipping account.

Designed for rapid, efficient, column-free extraction of PCR-ready DNA from C. elegans. Traditional extraction methods take 2 hours, the Worm Lysis Kit requires only a 15-minute incubation before the DNA is ready for use directly in your PCR.

DNA extraction is performed in a single tube without multiple washing steps to reduce potential contamination and sample loss and allows for quality DNA templates to be obtained consistently from single or multiple worm lysis reactions.

Benefits:

  •   Fast –Single-tube DNA extraction in 15 minutes
  •   Convenient - PCR-ready DNA
  •  Reliable - Maximized PCR speed, yield and specificity
  •  Consistent - Minimize contamination risks and sample loss

 

Advantages:

  •  Run multiple PCRs from a single lysis reaction.
  •  Each lysis reaction results in 100 uL of PCR-ready DNA and only 2 uL is required per PCR.
  •  Works well with as little as one worm per reaction or several worms.
  •  Amplicons of 3kb can be obtained by PCR, when DNA template is obtained from our lysis protocol.
  •  The DNA generated with the Worm Lysis Kit is suitable for use in all downstream PCR and qPCR applications without further clean-up steps. It can also be stored at -20°C for future use.

 

Customer Feedback:

"I was very skeptical that I could get a good signal from 2 ul of single worm lysate that was diluted to 100 ul. Well, I must admit it worked well! I was very surprised. For years we have been lysing single worms in 2-4 ul and using 2 ul per PCR reaction. I would have never guessed we could dilute our lysates to 100 ul and still get  a good signal from PCR. I think we would use it often". - Dr. Andy Golden, NIH

Worm Lysis kit

Product Info:

  • ●  Components: 5x Worm Lysis Buffer A (1 x 200 µl) and 10x Worm Lysis Buffer B (1 x 100 µl)
  • ●  Volume: each kit provides enough reagents for 100 lysates
  • ●  Storage upon receipt: <-20℃
Worm lysis kit

PCR amplification from single worm lysate. Single worms were picked into 10 µl of Worm Lysis Buffer. After the lysis protocol the samples were diluted 10-fold and used in a PCR reaction with the NemaMetrix PCR Mix.

  • - Stored at -20°C on arrival. Will retain full activity for 12 months at -20°C
  • - Avoid prolonged exposure to light.
  • - The kit can go through 30 freeze/thaw cycles with no loss of activity.