Sale!

Worm Lysis Kit

$95.00 $65.00

Worm Lysis Kit (Cat. No. PCR101) is designed for rapid, efficient, column-free extraction of PCR-ready DNA from C. elegans. DNA extraction is performed in a single tube without multiple washing steps to reduce potential contamination and sample loss.

Compared to the laborious and time consuming traditional extraction methods (min. 2 hours), our Worm Lysis Kit requires only a 15-minute incubation before the DNA is ready for use directly in your PCR and allows for quality DNA templates to be obtained consistently from single or multiple worm lysis reactions.

The DNA generated with the Worm Lysis Kit is suitable for use in all downstream PCR and qPCR applications without further clean-up steps. It can also be stored at -20°C for future use.

Benefits:

  •   Fast –Single-tube DNA extraction in 15 minutes
  •   Convenient – PCR-ready DNA
  •  Reliable – Maximized PCR speed, yield and specificity
  •  Consistent – Minimize contamination risks and sample loss

 

Advantages:

  •  Works well with as little as one worm per reaction or several worms
  •  Lysate can be diluted 10x for multiple PCR amplifications
  •  Amplicons of 3kb can be obtained by PCR, when DNA template is obtained from our lysis protocol
  •  Each kit provides enough reagents for 100 lysates.

 

Product Info:

  •  Components: 5x Worm Lysis Buffer A (1 x 200 µl) and 10x Worm Lysis Buffer B (1 x 100 µl)
  •  Storage upon receipt: <-20℃. 

 

Shipping Details:

  • – USA: $15 flat rate via USPS or use your own shipper account.
  • – International: $120 or use your own shipper account.

 

SKU: PCR101 Category:

Additional Product Information

Worm Lysis kit

PCR amplification from single worm lysate. Single worms were picked into 10 µl of Worm Lysis Buffer. After the lysis protocol the samples were diluted 10-fold and used in a PCR reaction with the NemaMetrix HS Red Taq PCR Mix.

Worm Lysis Kit

  • – Stored at -20°C on arrival. Will retain full activity for 12 months at -20°C
  • – Avoid prolonged exposure to light.
  • – The kit can go through 30 freeze/thaw cycles with no loss of activity.