C. elegans florescent reagents
 
Cat No. DYE9439

RediStain™ WormDye Lipid Green

$135.00

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RediStain™ WormDye Lipid Green is an intrinsically lipophilic fluorophore that can be used to effectively track lipids inside C. elegans. When using Lipid Green, native lipid droplet morphology is preserved and fat content per body volume can be quantified in individual worms.

The figure above shows comparison of C. elegans fat storage using 2 different dye staining techniques. Intestinal and epidermal fat in an adult C. elegans has been stained using RediStain™ WormDye Lipid Green (B) and Nile Red (C). It takes 20 minutes stain live worms using WormDye Lipid Green vs. 3 days using Nile Red on plates.

Product Info:
- Size: 500 uL (100 uses)
Packaged Concentration: 2 mg/mL
Solute: DMF
Storage upon receipt: <-20℃. Protect from light
Excitation/Emission: 549/565

C. elegans florescent reagents

Comparison of C. elegans fat storage using 2 different dye staining techniques. Intestinal and epidermal fat in an adult C. elegans has been stained using RediStain™ WormDye Lipid Green (B) and Nile Red (C). It takes 20 minutes to  to stain live worms using WormDye Lipid Green vs. 3 days using Nile Red on plates. 

RediStain™ WormDye Lipid Green

1. Klapper M. et al.  Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches. J. Lipid. Res. 52, 1281–1293 (2011).

2. Zhang S. O., Box A. C., Xu N., Le Men J., Yu J., et al. , 2010a Genetic and dietary regulation of lipid droplet expansion in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 107: 4640–4645.

3. Palgunow, D., Klapper, M., & Döring, F. (2012). Dietary Restriction during Development Enlarges Intestinal and Hypodermal Lipid Droplets in Caenorhabditis elegans. PLoS ONE, 7(11), e46198. http://doi.org/10.1371/journal.pone.0046198

4. Zhang, S. O., Trimble, R., Guo, F., & Mak, H. Y. (2010). Lipid droplets as ubiquitous fat storage organelles in C. elegans. BMC Cell Biology, 11, 96. http://doi.org/10.1186/1471-2121-11-96