Additional Product Information
LabTIE Instant OP50 dietary choice behavior in C. elegans
The video shows the preference of C. elegans for this freeze-dried OP50 as a convenient food source. Consistent quality enables reproducible results for experiments and technical replications. It is freeze-dried and has single cells when dissolved in liquid. The OP50 strain was acquired from CGC.
|Monoculture||Empty vials and pots were Gamma rayed 25 kiloGray (kGy), Monoculture OP50 was not Gamma rayed.|
|Density||± 5×109 cells when dissolved in 250mL|
|Solubility||Good – Color: Turbid yellow when dissolved in dH2O/S-medium|
|Cultivation methodology||Cultured in Super Optimal Broth (SOB) medium in a controlled environment under optimal conditions|
|Contents cultivation medium||Bacto-tryptone, Yeast extract, NaCl, KCL, MgCl2, MgSO4, Glycerol, Glucose|
Protocol Seeding Petri dishes (NGM agar)
- Amount to Dissolve
Dissolve the content of 1 vial containing 0.75 gram in 25 mL Sterile dH2O by vortexing the suspension thoroughly for 30 seconds. (equals 250 small and 100 medium Petri dishes to be seeded with ±5×1010 cells/mL)
- Amount to seed on a Petri dish
Apply 100 µL on a small Petri dish (60 mm) Apply 250 µL on a medium Petri dish (90 mm) Tip: circle the plate to spread out OP50 to speed up the drying process.
- Evaporation of dH2O
Dry the Petri dish for 15-60 minutes in a laminar flow hood.
- Seeding (two options)
C. elegans can be inoculated directly after the Sterile dH2O has been evaporated. Another option is to place the seeded Petri dish in an incubator for 12 hours at 37°C and inoculate C. elegans afterwards.
Protocol Inoculating 250mL Liquid Culture with OP50 (S-medium)
- Amount to Dissolve
Dissolve 0.75 gram (1 Vial) in 250 mL S-medium (1 liquid culture) using a magnetic stirrer. This will result in a concentration of ±5×109 cells/mL Or select an assay below to determine the innoculation amount of OP50 cells
Once the OP50 has been dissolved, C. elegans can be inoculated.
|Type of assay||Optimal OP50 CFU/mL||Experiment||Amount to dissolve in 250mL|
|Dietary restriction1, 2||5×1011
|Life Cycle & population growth rate4, 5||5×109
|Survival assay7||1 – 4×109||