WMicroTracker – 24/7 Automated Data Collection

The WMicroTracker provides the capability to measure the locomotor activity of nematodes including C. elegans and parasitic worms, cultured in multi-well plates. You can get detailed measurements of motion and viability, including path tracking, distance and speed measurements.

This system is also designed for high-throughput screening assays (Figure 1) to evaluate the biological effects of potential compounds/drugs, RNAi/morpholino, as well as for mutant phenotyping studies in small animals

With the WMicroTracker's 24/7 automated data collection capability, you can:

  • Automatically track and quantify worm movement (Figure 1).
  • Load your experiment, set your parameters and walk away. Your data will be ready for you at the end of the programmed time.
  • Perform live worm assays for several weeks at a time with high reproducibility of data.

Figure 1. The WMicrotracker ONE gives you data comparable to a thrashing assay, only 10 times faster. The WMicrotracker ONE quantifies the locomotor activity of a worm population in a 96 well plate with 30-70 animals per well. Data acquisition and analysis are automated and simultaneous. All activity is normalized to N2 controls.

WMicroTracker ONE demo video

A sample recording of animal path tracking in a 6-well microtiter plate using the WMicroTracker ONE device.

WMicroTracker Toxicity Assay

Figure 2. Toxicity assay. The dose-response effect of oxidative stress on C. elegans locomotor activity over time can be observed. Worms were exposed to a reactive oxygen species (ROS) generating compound. Each concentration was tested four times, using 30-70 animals per well in a 96-well plate.

Figure 3. Lifespan assay. The decreased lifespan of skn-1 mutants can be observed. Samples were recorded for 1 hour once per day, four times per condition.

WMicroTracker Chemotaxis Assay

Figure 4. Chemotaxis Assay. Worm chemotaxis toward benzaldehyde was automatically recorded. Three concentrations of benzaldehyde were placed on an agar substrate in a multiwell plate, using 30-70 animals per well.

Key Applications:

Key Advantages:

  • Automatically track & quantify worm movement
  • Long-term, consistent data acquisition
  • No user bias, no tedious training

Configurations:

The WMicroTracker instrument has 2 configurations:

  • WMicrotracker ONE, optimized for C. elegans grown in liquid media
  • WMicrotracker ARENA (to be released Dec. 2018, preorder now), optimized for C. elegans grown in solid media such as agar and with temperature stabilization.
Comparison of ONE vs. ARENA
CompatibilityONEARENA
Plates
6 wells
12 wells
24 wells
48 wells
96 wells
384 wells
Media
Liquid
Agar
Temperature
Monitoring
Control
ProductCat. No.Price
WMicroTracker ONEWTK100$15,000
WMicroTracker ARENAWTK200$19,000

Recent Publications

  1. Rescue of ATXN3 neuronal toxicity in C . elegans by chemical modification of ER stress. Fardghassemi Y ; Tauffenberger A ; Gosselin S ; Parker JA. Dis Model Mech. 2017 Dec 19;10(12):1465-1480.
  2. A rapid chemical ­genetic screen utilizing impaired movement phenotypes in C . elegans : Input into genetics of neurodevelopmental disorders. Schmeisser K ; Fardghassemi Y; Parker JA. Exp Neurol. 2017 Jul;293:101-114.
  3. Twitchin kinase inhibits muscle activity. Matsunaga Y ; Hwang H ; Franke B ; Williams R ; Penley M ; Qadota H ; Yi H ; Morran LT ; Lu H ; Mayans O ; Benian GM. Mol Biol Cell. 2017 Jun 15;28(12):1591-1600.
  4. Neurodegeneration in C. elegans models of ALS requires TIR-1/Sarm1 immune pathwayactivation in neurons. Veriepe J ; Fossouo L ; Parker JA. Nat Commun. 2015 Jun 10;6:7319.
  5. Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle. Matsunaga Y ; Qadota H ; Furukawa M ; Choe HH ; Benian GM. Mol Biol Cell. 2015 Jun 1;26(11):2096-111.
  6. Insulin/IGF-1 receptor signaling enhances biosynthetic activity and fat mobilization in the initialphase of starvation in adult male C. elegans. Tan KT ; Luo SC ; Ho WZ ; Lee YH. Cell Metab. 2011 Sep 7;14(3):390-402.