WMicroTracker – 24/7 Automated Data Collection

The WMicroTracker provides the capability to measure the locomotor activity of small nematodes such as C. elegans and related species, cultured in liquid media and in multi-well plates. You can get measurements of overall locomotor activity and viability of your worms.

This system is optimized for measuring C. elegans in liquid media and is also designed for high-throughput screening assays (Figure 1) to evaluate the biological effects of potential compounds/drugs, as well as for mutant phenotyping studies in small nematodes.

With the WMicroTracker's 24/7 automated data collection capability, you can:

  • Automatically measure worm movement in both large and small populations (Figure 1).
  • Perform live worm assays for several weeks at a time with high reproducibility of data.
  • Load your experiment, set your parameters and walk away. Your data will be ready for you at the end of the programmed time.

Figure 1. The WMicrotracker gives you data comparable to a thrashing assay, only 10 times faster. The WMicrotracker quantifies the locomotor activity of a worm population in a 96 well plate with 30-70 animals per well. Data acquisition and analysis are automated and simultaneous. All activity is normalized to N2 controls.

WMicroTracker demo video

A sample recording of animal path tracking in a 6-well microtiter plate using the WMicroTracker device.

WMicroTracker Toxicity Assay

Figure 2. Toxicity assay. The dose-response effect of oxidative stress on C. elegans locomotor activity over time can be observed. Worms were exposed to a reactive oxygen species (ROS) generating compound. Each concentration was tested four times, using 30-70 animals per well in a 96-well plate.

Figure 3. Lifespan assay. The decreased lifespan of skn-1 mutants can be observed. Samples were recorded for 1 hour once per day, four times per condition.

WMicroTracker Chemotaxis Assay

Figure 4. Chemotaxis Assay. Worm chemotaxis toward benzaldehyde was automatically recorded. Three concentrations of benzaldehyde were placed on an agar substrate in a multiwell plate, using 30-70 animals per well.

Key Advantages:

  • Automatically track & quantify worm movement
  • Long-term, consistent data acquisition
  • No user bias, no tedious training

Key Features:

  • Works with 96 and 384 well plate
  • Worms can be measured in liquid media
  • Automatic temperature monitoring

Parasitic Worms

The WMicroTracker can also be used to measure the locomotor activity of parasitic nematodes, cultured in liquid media and in multi-well plates. Users can get measurements of overall locomotor activity and viability of worms of various species and sizes.

Key advantages:

  • the only platform that combines label-free measurements, species flexibility, locomotor activity measurement in liquid media with high throughput.
  • measure species of nematodes in various sizes
  • can be placed directly inside an incubator for optimum temperature controls

Figure 4. Detect and measure E. Granulosus Protoscoleces movement using the WMicroTracker System . Movement is detected when a population more than 45 organisms/well is employed. Linear range is observed from 50 To 200 organisms/Well.

Comparision of WMicrotracker vs. ARENA
Application/FunctionWMicrotrackerARENA
Liquid Culture
Works with 96 and 384 well microplates
Fits in an incubator (for temp. control)
Toxicity Assays
Acute drug exposure
Stress Response
Agar
Path of the worm
Temperature control
Works with 35mm culture plates, 6, 24, and 48 well plates
Chemotaxis assays
LifeTime assays
Non-C. elegans worms
Requires multiple worms/plate or well
Temperature monitoring
ProductCat. No.Price
WMicroTrackerWTK100$15,000 (includes educational discount)

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