WMicroTracker – 24/7 Automated Data Collection

The WMicroTracker provides the capability to measure the locomotor activity of small nematodes such as C. elegans and related species, cultured in liquid media and in multi-well plates. You can get measurements of overall locomotor activity and viability of your worms.

This system is optimized for measuring C. elegans in liquid media and is also designed for high-throughput screening assays (Figure 1) to evaluate the biological effects of potential compounds/drugs, as well as for mutant phenotyping studies in small nematodes.

With the WMicroTracker's 24/7 automated data collection capability, you can:

  • Automatically measure worm movement in both large and small populations (Figure 1).
  • Perform live worm assays for several weeks at a time with high reproducibility of data.
  • Load your experiment, set your parameters and walk away. Your data will be ready for you at the end of the programmed time.

WMicroTracker demo video

The WMicroTracker gives you data comparable to a thrashing assay, only 10 times faster. The WMicroTracker quantifies the locomotor activity of a worm population in a 96 well plate with 30-70 animals per well. Data acquisition and analysis are automated and simultaneous. All activity is normalized to N2 controls.

WMicroTracker Toxicity Assay

Figure 2. Toxicity assay. The dose-response effect of oxidative stress on C. elegans locomotor activity over time can be observed. Worms were exposed to a reactive oxygen species (ROS) generating compound. Each concentration was tested four times, using 30-70 animals per well in a 96-well plate.

Figure 3. Lifespan assay. The decreased lifespan of skn-1 mutants can be observed. Samples were recorded for 1 hour once per day, four times per condition.

Key Advantages:

  • Automatically track & quantify worm movement
  • Long-term, consistent data acquisition
  • No user bias, no tedious training

Key Features:

  • Works with 96-well plate (Download the protocol)
  • Worms can be measured in liquid media
  • Automatic temperature monitoring

Parasitic Worms

The WMicroTracker can also be used to measure the locomotor activity of parasitic nematodes, cultured in liquid media and in multi-well plates. Users can get measurements of overall locomotor activity and viability of worms of various species and sizes.

Key advantages:

  • the only platform that combines label-free measurements, species flexibility, locomotor activity measurement in liquid media with high throughput.
  • measure species of nematodes in various sizes
  • can be placed directly inside an incubator for optimum temperature controls

Figure 4. Detect and measure E. granulosus protoscoleces movement using the WMicroTracker System. Movement is detected when a population of more than 45 organisms/well is employed. Linear range is observed from 50 to 200 organisms/well for a 30-minute observation period.

WMicrotracker Features
Liquid Culture
Works with 96 and 384 well microplates (Greiner)
Fits in an incubator (for temp. control)
Toxicity Assays
Acute drug exposure
Stress Response
Requires multiple worms/plate or well
Temperature monitoring
ProductCat. No.Price

Recent Publications

C. elegans

  1. C. elegans Development and Activity Test Detects Mammalian Developmental Neurotoxins. Piper Reid Hunt; Nicholas Olejnik; Keenan D. Bailey; Cory A. Vaught; Robert L. Sprando. 2018 Nov; 121:583-592
  2. Active principles of Tetradenia riparia. IV. Anthelmintic activity of 8(14),15-sandaracopimaradiene-7α,18-diol. Luc Van Puyvelde; Maoxuan Liu; Cedrick Veryser; Wim M. De Borggraeve; Joseph Mungarulire; Marie Jeanne Mukazayire; Walter Luyten. 2018 Apr; 216:229-232
  3. Rescue of ATXN3 neuronal toxicity in C . elegans by chemical modification of ER stress. Fardghassemi Y ; Tauffenberger A ; Gosselin S ; Parker JA. Dis Model Mech. 2017 Dec 19;10(12):1465-1480.
  4. A rapid chemical ­genetic screen utilizing impaired movement phenotypes in C . elegans : Input into genetics of neurodevelopmental disorders. Schmeisser K ; Fardghassemi Y; Parker JA. Exp Neurol. 2017 Jul;293:101-114.
  5. Twitchin kinase inhibits muscle activity. Matsunaga Y ; Hwang H ; Franke B ; Williams R ; Penley M ; Qadota H ; Yi H ; Morran LT ; Lu H ; Mayans O ; Benian GM. Mol Biol Cell. 2017 Jun 15;28(12):1591-1600.
  6. Neurodegeneration in C. elegans models of ALS requires TIR-1/Sarm1 immune pathwayactivation in neurons. Veriepe J ; Fossouo L ; Parker JA. Nat Commun. 2015 Jun 10;6:7319.
  7. Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle. Matsunaga Y ; Qadota H ; Furukawa M ; Choe HH ; Benian GM. Mol Biol Cell. 2015 Jun 1;26(11):2096-111.
  8. Insulin/IGF-1 receptor signaling enhances biosynthetic activity and fat mobilization in the initialphase of starvation in adult male C. elegans. Tan KT ; Luo SC ; Ho WZ ; Lee YH. Cell Metab. 2011 Sep 7;14(3):390-402.

Parasitic Worms

  1. Bioassay-guided isolation of three anthelmintic compounds from Warburgia ugandensis Sprague subspecies ugandensis, and the mechanism of action of polygodial. Liu M, Kipanga P, Mai AH, Dhondt I, Braeckman BP, De Borggraeve W, Luyten W.  Int J Parasitol. 2018 Sep;48(11):833-844. 
  2. Screening of a drug repurposing library with a nematode motility assay identifies promising anthelmintic hits against Cooperia oncophora and other ruminant parasites. Liu M., Landuyt B., Klaassen H., Geldhof P., Luyten W. Veterinary Parasitology. 2018 Dec. 
  3. The nervous and prenervous roles of serotonin in Echinococcus spp. Camicia F, Herz M, Prada LC, Kamenetzky L, Simonetta SH, Cucher MA, Bianchi JI, Fernández C, Brehm K, Rosenzvit MC. Int J Parasitol. 2013 Jul;43(8):647-59.
  4. Unique pharmacological properties of serotoninergic G-protein coupled receptors from cestodes. Rosenzvit MC et al. PLoS Negl Trop Dis. 2018 Feb; 12(2): e0006267.

Technical Resources