The ScreenChip™ System

Synchronize and Associate Visual Phenotypes with Feeding Behavior

To understand the mechanisms of action of your proteins or drugs, it is important to understand the behavior of overall worm population as well as phenotypes that reveal the underlying cellular and molecular forces in action. The ScreenChip™ System, along with the WMicrotracker System allow you to get in-depth understanding of your worm behavior.

The ScreenChip™ System, a C. elegans visual screening and phenotyping platform that allows you to associate visual phenotypes with feeding behavior.

  • Measure, visualize and analyze the neuromuscular aspects of feeding behavior
  • Assess the neuronal and physiological responses to drugs, aging, genetic modifications or environmental changes (Fig. 1 & 2)
  • Perform multiple phenotyping analyses at once, i.e morphology, fluorescence, defecation, and feeding behavior while your worm in immobilized in the chip
  • Eliminate experimenter’s bias and get more reproducible data

ScreenChip System Overview

EPG and movement visual synchronization

Data output from the ScreenChip System

NemaMetrix ScreenChip Cartridges, Dock, and Amplifier

ScreenChip Docking Station

Applications

Aging

Aging phenotypes observed as changes in EPG trace

Pumping Regularity Decreases with Age

  • Aging nematodes exhibit irregularity in inter-pump intervals (below).
  • No significant change is observed in pump duration upon nematode aging (data not shown).

Pump amplitude increases with age

  • Nematodes exhibit a higher pump   amplitude with age (below); this is   concomitant with the larger, stronger   pharynx of developed adult worms.
  • Average pump frequency is observed   to decrease with nematode age (below).

Anti-aging and lifespan effects are quantifiable

  • Changes in nematode pump frequency recapitulate the effects of age-associated decline
  • Trehalose treatment mitigates the effects of ageing, in support of prior work reporting that trehalose extends nematode lifespan

Disease Modeling

Humanized worm recapitulates function and physiology

A Customized C. elegans Model for Epilepsy

  • Comparison of the human STXBP1 sequence to the worm’s unc-18 gene exhibits only 59% identical amino acid usage
  • Disease variant biology more accurately modeled by focusing on gene-swap humanization rather than the native animal locus
  • Sequence optimized human cDNA inserted at start codon of worm unc-18; all endogenous coding removed, transgene uses native 3' UTR

Humanized C. elegans Shows Functional Rescue

  • Pharynx pumping trace exhibits full loss of WT pumping activity in STXBP1 ortholog unc-18 KO (below)
  • Gene-swap of hSTXBP1 at the worm unc-18 locus shows functional rescue in pharyngeal pumping phenotype

Conservation of Physiology in the Humanized Worm

  • 79% of disease genes are conserved between human and worm
  • STBXP1 is ranked as the #6 likely genetic cause of epilepsy; of the 349 variants, 47 are pathogenic (13%)
  • Rescued function and physiology paves the way for screening pathogenic gene variants

Compound Testing

Visualize whole-worm pharmocological effects via EPG

Reported Compound Effects Recapitulated in EPG Analysis

  • Inhibitory neuromuscular effects of Ivermectin (IVM) are observed as an abrupt loss of   pharyngeal pumping activity (right)
  • No effect on neuromuscular activity is observed   in worms experiencing a mock-drug treatment
  • IVM resistant nematodes exhibit the expected pharmacological effect of persistent partial activity
EPG Recordings from Single Device
EPG Recordings from Single Device

Compound Treatment Results in Quantifiable EPG Changes

  • EPG traces exhibit shifts in observed amplitude, frequency, and duration upon exposure to IVM (right; pre-treatment in black, 35min treatment in blue)
  • EPG traces can be analyzed for multiple parameters in parallel; effects of candidate compounds can be distinctly characterized
Probability Distribution

The ScreenChipTM System can also be used in the field of toxicology to quantify adverse effects of chemical substances (e.g., environmental pollutants, drugs) on living organisms. Learn more about toxicology testing.

With the ScreenChip System, you can:

  • Record videos that are perfectly synchronized to the EPG traces, all with a single software.
  • Observe and playback all the molecular and cellular events in the pharynx to better understand what is happening to your worms.

All you need is a microscope (inverted or dissection), a vacuum source and a computer that meets the minimum requirements for running the ScreenChip Software.

Please ensure that your microscope includes the following basic requirements:

  • a C-mount or CS-mount camera port
  • For magnifications 10X or 20X: inverted  
  • For 40X magnification: standard long working distance objective (higher magnification not suitable with the ScreenChip system)
  • Cold light source (LED)

Fluorescence: The ScreenChips are 100% optically clear and compatible with all light wavelengths (excitation and acquisition).

ScreenChip System with visual synchronization

What we use in our lab:

  • Leica M165 FC - Fluorescent dissecting microscope
  • Zeiss Axiovert - High magnification (40X and up) inverted microscope

We haven't tested other brands and models, however microscopes with similar specifications should work well with the ScreenChip system.

Simple wash-and-load sample preparation

- No mounting and single-worm manipulation
- No previous C. elegans experience required
- Automated data collection and analysis

Four steps to feeding behavior data

ScreenChip System 4 steps to data
ProductUnitCat. No.
ScreenChip System1SKC101A, SKC102A
ScreenChip CartridgesBox of 10SC10, SC20, SC30, SC40, SC60, SCX1, SX99
Worm Recovery Kit1WREC01
Vacuum Pump1VAC100

ScreenChip System Publications