The ScreenChip™ System

Synchronize and Associate Visual Phenotypes with Feeding Behavior

To understand the mechanisms of action of your proteins or drugs, it is important to understand the behavior of overall worm population as well as phenotypes that reveal the underlying cellular and molecular forces in action. The ScreenChip™ System, along with the WMicrotracker System allow you to get in-depth understanding of your worm behavior.

The ScreenChip™ System, a C. elegans visual screening and phenotyping platform that allows you to associate visual phenotypes with feeding behavior.

  • Measure, visualize and analyze the neuromuscular aspects of feeding behavior
  • Assess the neuronal and physiological responses to drugs, aging, genetic modifications or environmental changes (Fig. 1 & 2)
  • Perform multiple phenotyping analyses at once, i.e morphology, fluorescence, defecation, and feeding behavior while your worm in immobilized in the chip
  • Eliminate experimenter’s bias and get more reproducible data
EPG and movement visual synchronization
NemaMetrix ScreenChip Cartridges, Dock, and Amplifier
Mean pump frequency in C elegans - Anandamide and controls.

Figure 1.  The cannabinoid Anandamide induces hyperphagia in adult worms (red dots). Mean pump frequency versus time in 10 sec. bins (Anandamide, N = 27; Control, N = 41). : Data are shown as mean ± SEM. ANOVA, Treatment, F(1,66) = 7.26, p = 0.009.

Figure 2. Comparison of adult C. elegans worms within the ScreenChip system. Day 1 and Day 13 worms were recorded in a 40 um and 60 um channels respectively. Recording pharyngeal activity uncovered a drastic change in feeding activity, not only in frequency but also in feeding pattern and quality.

With the ScreenChip System, you can:

  • Record videos that are perfectly synchronized to the EPG traces, all with a single software.
  • Observe and playback all the molecular and cellular events in the pharynx to better understand what is happening to your worms.

All you need is a microscope (inverted or dissection), a vacuum source and a computer that meets the minimum requirements for running the ScreenChip Software.

Please ensure that your microscope includes the following basic requirements:

  • a C-mount or CS-mount camera port
  • For magnifications 10X or 20X: inverted  
  • For 40X magnification: standard long working distance objective (higher magnification not suitable with the ScreenChip system)
  • Cold light source (LED)

Fluorescence: The ScreenChips are 100% optically clear and compatible with all light wavelengths (excitation and acquisition).

ScreenChip System with visual synchronization

What we use in our lab:

  • Leica M165 FC - Fluorescent dissecting microscope
  • Zeiss Axiovert - High magnification (40X and up) inverted microscope

We haven't tested other brands and models, however microscopes with similar specifications should work well with the ScreenChip system.

Simple wash-and-load sample preparation

- No mounting and single-worm manipulation
- No previous C. elegans experience required
- Automated data collection and analysis

Four steps to feeding behavior data

ScreenChip System 4 steps to data
ProductUnitCat. No.
ScreenChip System1SKC101A, SKC102A
ScreenChip CartridgesBox of 10SC10, SC20, SC30, SC40, SC60, SCX1, SX99
Worm Recovery Kit1WREC01
Vacuum Pump1VAC100