No more experiment delays! Harvest synchronized L1 C. elegans without bleaching, chemicals, or starvation in 15 minutes.

The Celegans Synchronization System (CES) is a manual worm Synchronizer that allows you to achieve near absolute synchronization – a 99.9% L1 purity in your L1 harvest with no bleach or chemicals, and with little or no training required. You get healthy worms while increasing the reproducibility of your data. 

With the C. elegans Synchronization System, you can:

  • harvest small and large volumes of nearly 100% synchronized L1s from a mixed worm population without the use of chemicals or starvation. 
  • get synchronized worms that do not contain phenotypes caused by bleach or food arrest. 
  • eliminate the need for worm controls and variable outcomes.

Figures: Results showing an average of 99.9% purity of L1’s when using the C. elegans Synchronization System.  A 50mL F3 generation S-medium culture was used, containing a C. elegans mixed population and was synchronized in accordance with the protocol. A 90 minutes harvest time was used in S-media without OP50. (A) After synchronizing, the total amount of worms were counted from 10 harvesting experiments.  (B) Using the C. elegans Synchronizer, the amount of L1’s present in the harvest samples compared to other stages (anomalies) was identified to determine the accuracy of the Synchronizer. Anomalies were counted in each harvesting experiment. Results were plotted against the total amount of worms. Example harvesting experiment 1: 6400 L1 worms vs 10 anomalies (7 x L2's, 3 x L3's) = 100-(100/6400*10) = 99.84% L1 purity in harvest compared to other stages present.

Key advantages:

  • Healthy worms, larger L1 yield, highly reproducible data (Fig. A & B)
  • Phenotype free worms synchronized without bleach, chemicals and starvation
  • Improved level of synchronization with little variation (Fig. c)
  • Achieve consistent results with little or no training required.

Figure A. Results showing the reproducibility of the CES to generate synchronous L1 population across 34 replicates. L1 worms were injected and cultivated on chips during 5 days. Each channel (represented on the x-axis) corresponds to 3 to 8 microfluidic chambers (example on the right side of this panel) containing 1 to 4 worms. Each dots represented on the graphic correspond to the timing (in hours) when the first egg is observed in average in the corresponding channel. The error bars represent the standard deviation.

Figure B. Comparison of the percentage of fertile worms between three methods of L1 synchronization. Each bar represented on the graphic correspond to the percentage of chambers (on average) with fertile adult worms. n corresponds to the number of chambers analyzed. The error bars represent the 95% CI.

Figure C. Variation of the timing to reach the adult stage across single individuals. Each dot represented on the graphic correspond to the variation of the timing for a single worms to reach the adult stage, compared to the whole population analyzed. n corresponds to the number of single worms analyzed. sd corresponds to the value of the standard deviation. All worms reach adulthood virtually at the same time.

How does it work?

The L1 Celegans Synchronizer is a 2-step filtration system harvesting freshly hatched L1s.

Step 1: Wash and stabilizing filter. Wash out all the debris and anything smaller than Adults and eggs. Only adults and eggs are kept.

Step 2: Harvest filter and synchronization. Adults and eggs will be transferred in 'real time' as the L1 hatchlings will transfer through a filter with little or no delay.

Frequently Asked Questions

Preferred method is to use 0.5M NaOH and / or (40khz) small ultrasonic cleaner.   The ultra-sonic cleaner will ensure the filters are truly clean, while speeding up the process of cleaning. This will typically also take care of scaling.  It is not recommended to descale the filters using an acid solution.  Using an acid will at a minimum cause coloring/stains on the filter and repeated use of acids will ultimately damage the filters.

Yes, you can use small volumes of a worm culture. Recommended is a minimum of 10 gravid adults including laid eggs, however larger volumes have also been successfully processed in a single batch.

Yes, each strain is synchronized in approximately 10 to 15 minutes. Please keep in mind that each strain will have to remain in the 'Harvest Filter' for a period of time (i.e. 'Harvest Time'). In order to synchronize multiple strains and not have to wait for the Harvest Filter become available again, it is advised to purchase for each additional strain an extra 'Harvest Filter'. This will allow extended harvest time for each strain. In a typical setup, where one person is working with the Synchronizer System, one (1) stabilizing filter should suffice. The Stabilizing Filter is used in the first step of the protocol and becomes available as soon as its content is transferred to the Harvest Filter. Depending on the on the need to exclude  cross-contamination, the Stabilizer Filter may need to be cleaned / rinsed before processing the next strain.

Unlike the traditional filters, the CES filters do not have a 'round' hole or 'semi-round' holes (aperture) so nematodes cannot swim through. This design also prevent the filters from clogging when a user tries to filter a large amount of a mixed population of worms. The filter design requires a very tight margin tolerance and an absolute zero defect of every single aperture in order to get nearly perfect synchronization.

Ordering Information

NemaMetrix sells this product only in North America. Please contact NemaSync if you are outside North America.

SKUDescriptionPrice
CES-V2C. elegans Synchronizer (CES)$4,895.00
FSCE-01Stabilizing Filter$2,550.00
FHCE-01Harvest Filter$2,550.00
CESFH-01Funnel + Holder + Tube$485.00
CESF-SP-01Funnel (Spare Part) $375.00
CESFH-SP-01Funnel Holder (Spare Part) $185.00
CESHR-SP-01Funnel Holder Separator Ring (Spare Part)$75.00