The C. elegans Synchronization System (CES) is a manual worm Synchronizer that allows you to achieve near absolute synchronization – a 99.9% L1 purity in your L1 harvest with no bleach or chemicals, and with little or no training required. You get healthy worms while increasing the reproducibility of your data. With the C. elegans Synchronization System, you can: Figures: Results showing an average of 99.9% purity of L1’s when using the C. elegans Synchronization System. A 50mL F3 generation S-medium culture was used, containing a C. elegans mixed population and was synchronized in accordance with the protocol. A 90 minutes harvest time was used in S-media without OP50. (A) After synchronizing, the total amount of worms were counted from 10 harvesting experiments. (B) Using the C. elegans Synchronizer, the amount of L1’s present in the harvest samples compared to other stages (anomalies) was identified to determine the accuracy of the Synchronizer. Anomalies were counted in each harvesting experiment. Results were plotted against the total amount of worms. Example harvesting experiment 1: 6400 L1 worms vs 10 anomalies (7 x L2's, 3 x L3's) = 100-(100/6400*10) = 99.84% L1 purity in harvest compared to other stages present.
The C. elegans Synchronization System (CES) is a manual worm Synchronizer that allows you to achieve near absolute synchronization – a 99.9% L1 purity in your L1 harvest with no bleach or chemicals, and with little or no training required. You get healthy worms while increasing the reproducibility of your data.
With the C. elegans Synchronization System, you can:
Figures: Results showing an average of 99.9% purity of L1’s when using the C. elegans Synchronization System. A 50mL F3 generation S-medium culture was used, containing a C. elegans mixed population and was synchronized in accordance with the protocol. A 90 minutes harvest time was used in S-media without OP50. (A) After synchronizing, the total amount of worms were counted from 10 harvesting experiments. (B) Using the C. elegans Synchronizer, the amount of L1’s present in the harvest samples compared to other stages (anomalies) was identified to determine the accuracy of the Synchronizer. Anomalies were counted in each harvesting experiment. Results were plotted against the total amount of worms. Example harvesting experiment 1: 6400 L1 worms vs 10 anomalies (7 x L2's, 3 x L3's) = 100-(100/6400*10) = 99.84% L1 purity in harvest compared to other stages present.
- Healthy worms, larger L1 yield, highly reproducible data (Fig. A & B)
- Phenotype free worms synchronized without bleach, chemicals and starvation
- Improved level of synchronization with little variation (Fig. c)
- Achieve consistent results with little or no training required.
Figure A. Results showing the reproducibility of the CES to generate synchronous L1 population across 34 replicates. L1 worms were injected and cultivated on chips during 5 days. Each channel (represented on the x-axis) corresponds to 3 to 8 microfluidic chambers (example on the right side of this panel) containing 1 to 4 worms. Each dots represented on the graphic correspond to the timing (in hours) when the first egg is observed in average in the corresponding channel. The error bars represent the standard deviation.
Figure B. Comparison of the percentage of fertile worms between three methods of L1 synchronization. Each bar represented on the graphic correspond to the percentage of chambers (on average) with fertile adult worms. n corresponds to the number of chambers analyzed. The error bars represent the 95% CI.
Figure C. Variation of the timing to reach the adult stage across single individuals. Each dot represented on the graphic correspond to the variation of the timing for a single worm to reach the adult stage, compared to the whole population analyzed. n corresponds to the number of single worms analyzed. sd corresponds to the value of the standard deviation. All worms reach adulthood virtually at the same time.
How does it work?
The L1 C. elegans Synchronizer is a 2-step filtration system harvesting freshly hatched L1s.
Step 1: Wash and stabilizing filter. Wash out all the debris and anything smaller than Adults and eggs. Only adults and eggs are kept.
Step 2: Harvest filter and synchronization. Adults and eggs are transferred from the Stabilizing filter to the Harvest filter. As soon as the eggs hatch, the L1's will transfer through the filter with little or no delay.
Frequently Asked Questions
Preferred method is to use 0.5M NaOH and / or (40khz) small ultrasonic cleaner. The ultra-sonic cleaner will ensure the filters are truly clean, while speeding up the process of cleaning. This will typically also take care of scaling. It is not recommended to descale the filters using an acid solution. Using an acid will at a minimum cause coloring/stains on the filter and repeated use of acids will ultimately damage the filters.
Yes, you can use small volumes of a worm culture. Recommended is a minimum of 10 gravid adults including laid eggs, however larger volumes have also been successfully processed in a single batch.
Yes, each strain is synchronized in approximately 10 to 15 minutes. Please keep in mind that each strain will have to remain in the 'Harvest Filter' for a period of time (i.e. 'Harvest Time'). In order to synchronize multiple strains and not have to wait for the Harvest Filter become available again, it is advised to purchase for each additional strain an extra 'Harvest Filter'. This will allow extended harvest time for each strain. In a typical setup, where one person is working with the Synchronizer System, one (1) stabilizing filter should suffice. The Stabilizing Filter is used in the first step of the protocol and becomes available as soon as its content is transferred to the Harvest Filter. Depending on the on the need to exclude cross-contamination, the Stabilizer Filter may need to be cleaned / rinsed before processing the next strain.
Unlike the traditional filters, the CES filters do not have a 'round' hole or 'semi-round' holes (aperture) so nematodes cannot swim through. This design also prevent the filters from clogging when a user tries to filter a large amount of a mixed population of worms. The filter design requires a very tight margin tolerance and an absolute zero defect of every single aperture in order to get nearly perfect synchronization.
The C. elegans Synchronizer System comes with a limited warranty for manufacturing defects and in case it has been damaged in transit. However we cannot accept ‘Products for Return & Restocking’ as the ‘Main Filters’ cannot be refurbished once being used, as is typically the case for any other brand/type of filter in our industry.
Please find below our limited Warranty and Return Policy, we hope you never have to refer to.
1.1. Subject to the terms and conditions set out below, NemaSync, the manufacturer of the device, located in the Netherlands, agrees to repair or replace the Product at its own cost and any NemaSync accessory supplied with it, purchased by Customer from NemaMetrix Inc., in circumstances where the Product does not perform due to a manufacturing defect for a period of 24 months, commencing on the date of delivery (or deemed delivery) of the Product.
1.2. The Customer is responsible to inspect all Products received from NemaMetrix Inc. upon arrival. In instances where Products have been damaged in transit, the Customer must report this to NemaMetrix Inc.within 5 working days of receipt of the product. Failure to report physical damage on arrival within 5 working days of receipt may result in denial of warranty for physical damage.
2. Return Policy:
2.1. Products are being sold “as is” and in principle can NOT be returned when used.
2.1.1. In principle Products can only be returned in case:
220.127.116.11. Product(s) being damaged in transit (see also 1.2),
18.104.22.168. It has been concluded by NemaSync, that the Product has a manufacturing defect.
2.2. A return authorization must be requested and approved beforehand by NemaMetrix. To request a return authorization, please email firstname.lastname@example.org.
2.3. For incomplete warranty/return requests, NemaMetrix Inc. reserves the right to refuse any compensation or replacement of Product. If the Customer has returned the package without prior authorization, sends to the wrong address, returns an incomplete item or Product, or submits an empty package, then again NemaMetrix Inc. reserves the right to refuse any compensation.
2.4. If a Product is authorized to be returned, the Product(s) needs to be returned preferably in the original packaging and the return shipping cost to be paid by customer (‘DAP’ shipping – Incoterms 2010).
2.5. Customer has a duty of care and the Product needs to be returned in its original condition.
2.6. When the returned Product is received in good order by NemaMetrix Inc. and in accordance with the terms and conditions as defined in this agreement, NemaMetrix Inc. will either replace the Product with a new Product or reimburse the purchase value.
3. Taxes and shipping cost :
3.1. The Shipping fee for returning the product will be paid by the customer and is non-refundable.
3.2. Any Taxes and/or Import duties arising from import/export of Products shall be paid by Customer. In the case of a return shipment, we will deduct the customs fees and/or taxes from your refund amount.
3.3. In case of a warranty return, where the manufacturer replaces or repairs the Product, NemaMetrix shall pay shipping cost to Customer under ‘DAP’ shipping terms.
4. Disclaimer of Warranty; Due Diligence and Limitation of Liability:
4.1. To the full extent permitted by law :
4.1.1. Disclaimer of Warranty; Due Diligence. The Products are being sold “as is,” and NemaMetrix Inc. disclaims all warranties of quality, whether express or implied, including the warranties of merchantability and fitness for particular purpose. The Customer acknowledges that it has not been induced by any statements or representations of any person with respect to the quality or condition of the Products and that no such statements or representations have been made. The Customer acknowledges that it has relied solely on the investigations, examinations, and inspections as the Customer has chosen to make.
4.1.2. Limitation of Liability. NemaMetrix will not be liable for any indirect, special, consequential, or punitive damages (including lost profits) arising out of or relating to the Products or the transactions it contemplates (whether for breach of contract, tort, negligence, or other form of action) and irrespective of whether NemaMetrix has been advised of the possibility of any such damage. In no event will NemaMetrix’s liability exceed the price the Customer paid to NemaMetrix for the specific Products provided by NemaMetrix giving rise to the claim or cause of action.
NemaMetrix sells this product only in North America. Please contact NemaSync if you are outside North America.
|CES-V2||C. elegans Synchronizer (CES)||$4,895.00|
|CESFH-01||Funnel + Holder + Tube||$485.00|
|CESF-SP-01||Funnel (Spare Part)||$375.00|
|CESFH-SP-01||Funnel Holder (Spare Part)||$185.00|
|CESHR-SP-01||Funnel Holder Separator Ring (Spare Part)||$75.00|