In 2017, a now-redacted paper published in Nature Methods claimed that CRISPR created hundreds of unintended mutations in mouse models, and that algorithms designed to detect those “off target effects” weren’t working. The news sent share prices of CRISPR-based companies plummeting, and created rumblings—even panic—among scientists and gene editing-watchers worldwide. How accurate was CRISPR-Cas9, after all?
CRISPR/Cas9-mediated integration of loxP sequences for conditional mutagenesis in zebrafish has recently made its way into the toolkit of zebrafish researchers. In particular, CRISPR/Cas9 mutagenesis coupled with ssODN-mediated repair has simplified experimental design and provided a robust framework for conditional mutagenesis, as readily demonstrated by several groups in the zebrafish community to date….
In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of which the genome has been sequenced completely. One surprising result of this approach was that ~65% of the human disease genes have a counterpart in the worm….
Did you know April 25th is National DNA Day? This day is held annually to celebrate the completion of the Human Genome Project in 2003 and the discovery of DNA’s double helix in 1953, two discoveries that have paved the way for the amazing progress being made with genome engineering today. As the history of genome engineering […]
Researching chemicals that may cause damage to the nervous system is very challenging—of the 1,000 known toxic chemicals, only a dozen have been found to be toxic, but many unknowns exist. While mammal models remain the primary selection of research labs looking at the effects of metals and certain organic molecules on the brain—especially the […]