Simple. Convenient. Reliable.
Don’t settle for the inconsistency of in-house grown OP50. Use Freeze-dried Instant OP50 from LabTIE to obtain reproducible food quality and lawn thickness. Freeze-dried Instant OP50 is in ready-to-use format to trigger steady feeding behavior over time and enables you to:
- Remove the variable of ‘quality of food/nutrition’ from the equation in your experiments
- Feed C. elegans with a specific and measurable amount of nutrition
- Eliminate the time and effort needed to grow your own OP50
Instant OP50 from LabTIE is a high quality cultured Escherichia coli and is used as food source for the nematode Caenorhabditis elegans. It is used to seed NGM agar plates or add to liquid cultures (S-medium).
- High and consistent quality
- Direct use of NGM Petri dishes after innoculation
- Empty vial is Gamma-irradiated, OP50 is not
- Freeze-dried (easy storage)
- Compatible with microfluidic chips
LabTIE Instant OP50 dietary choice behavior in C. elegans
The video shows the preference of C. elegans for this freeze-dried OP50 as a convenient food source. Consistent quality enables reproducible results for experiments and technical replications. It is freeze-dried and has single cells when dissolved in liquid. The OP50 strain was acquired from CGC.
Example Data and Comparison
Freeze-dried Instant OP50 triggers steady feeding behavior over time. Pharyngeal pumping frequency over time as elicited in Day-1 adult C. elegans. Worms were starved for 2 hours on a plate prior to 7-min incubation in re-hydrated freeze-dried bacteria (1.52mg/mL). n = 4 to 9 worms per time point.
|Monoculture||Empty vials and pots were Gamma rayed 25 kiloGray (kGy), Monoculture OP50 was not Gamma rayed.|
|Density||± 5×109 cells when dissolved in 250mL|
|Solubility||Good – Color: Turbid yellow when dissolved in dH2O/S-medium|
|Cultivation methodology||Cultured in Super Optimal Broth (SOB) medium in a controlled environment under optimal conditions|
|Contents cultivation medium||Bacto-tryptone, Yeast extract, NaCl, KCL, MgCl2, MgSO4, Glycerol, Glucose|
|Description||Qty||Seed Vol.*||Plates*||Packaging||Price||Cat. No.|
|Freezedried OP50 Trial Vial||5 vials||5x 250 mL||500||$149.95||OP50-31772|
|Freezedried OP50 20 units||20 vials||20x 250 mL||2,000||$359.99||OP50-31773|
|Freezedried OP50 bulk 40 units||1 bottle||40x 250 mL||4,000||$611.99||OP50-31775|
|Freezedried OP50 bulk 100 units||1 bottle||100x 250 mL||10,000||$1,475.99||OP50-31776|
* 1 unit/vial will seed 250 mL liquid culture or 100 plates.
Note: We are the exclusive distributor for this product in North America. We can only sell to US or Canada customers. If you are outside North America, please contact LabTie directly.
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