What the ScreenChip System is:
- The ScreenChip System is comprised of a recording dock; compact amplifier; associated cables, tubing and connectors; ScreenChips; and NemAquire software.
- The ScreenChip System is used with standard lab equipment: a microscope, a vacuum source and a computer (Mac or PC).
How to set it up:
- Setup takes less than ten minutes. Easy-to-follow instructions guide you in setting up your system.
- The system has a small footprint and will comfortably fit on dissecting or inverted microscope stages.
How the system works:
- With the push of a button, the ScreenChip System gently moves worms one-by-one from an Eppendorf tube into a recording chamber.
- Electrodes in the ScreenChip record the voltage signals produced by each pharyngeal pump (the EPG).
- The signals are displayed in NemAquire software.
How to use the system:
- Experimental design is limited only by your creativity! You can compare different genotypes (e.g., wild-type and mutant); screen compounds against C. elegans human disease models, including aging; characterize drug pharmacology; analyze RNAi effects, and the list goes on.
- The operator determines how many worms to record from, and the duration of each recording.
- For example, in 1 day, an operator can make 2-minute recordings from 100 worms. This can represent up to ~50,000 pumps, providing exceptional statistical power for distinguishing subtle phenotypes.
- Each recording is saved as a separate data file, with user-entered experimental notes.
How to analyze the data:
- Data files are saved with a .txt extension and can be uploaded to the analysis platform of your choice (MATLAB, IGOR, LabChart, etc).
- For those with limited analysis experience, NemaMetrix offers a free and quick turn-around analysis service. You upload your data, and we return basic EPG parameters such as pump frequency and amplitude. Automated analysis software is coming soon.
- EPG recordings produce publishable data at an astonishing rate.