At our inaugural ScreenChip Workshop, one of our very first customers, Dr. Mike Harris, had a burning question.
He’d been collaborating for years on a project that focused on the rabies virus surface glycoprotein (RGP), and through careful characterization, he had found a conserved peptide sequence in the RGP that bore homology to sequences found in snake toxins which inhibit nicotinic acetylcholine receptors (nAChRs).
But he needed to know: Does the RGP peptide also function to inhibit nAChRs within the central nervous system in an in vivo assay?
To answer his question, he turned to C. elegans and the ScreenChip System. During the workshop, we helped Mike to test his hypothesis both through microinjections of the peptide and through a peptide bath application protocol. Excitement rippled through the room as Mike, just a day after being introduced to our system, produced publishable data that showed a marked decrease in pharyngeal pumping following exposure to the RGP peptide. He’ll be presenting this data at this year’s Experimental Biology conference in San Diego, April 2-6.
Pharyngeal pumping is a physiological process that is dependent on nAChR signaling, and thus provides a powerful readout of perturbations to this biochemical pathway. Our system records the strong electrical signal from the pharynx quickly and with minimal effort. It provides you with meaningful, information dense data in minutes.
We were amazed and honored to be a part of Mike’s discovery. Be sure to check out his poster if you are at Experimental Bio this year, and check out his abstract below!
Presentation Time: Monday, 12:45 – 3:00 pm.
Program #: 969.2
Location: B296 969.2
Title: Screening Bioactivity of Virus Surface Glycoprotein Peptides using C. elegans Electropharyngeogram
The next ScreenChip WorkShop is on April 26th and we’d love for you to join us. As always, NemaMetrix will cover the cost of your food and lodging for the duration of the workshop. Email today to set up your experiments and reserve your spot. We can’t wait to see what new projects scientists will bring to life in our lab.