Do Nematode Worms get 'The Munchies’? | Shawn Lockery, Ph.D. | The NemaMetrix ScreenChip System is a microfluidics platform for recording electropharyngeograms (EPGs) from the nematode worm Caenorhabditis elegans and other nematode species. This technical note demonstrates the utility of the ScreenChip system in pharmacology, taking as an example compounds chemically related to Δ9-tetrahydrocannabinol (THC), the primary psychoactive compound in marijuana (Cannabis sativa).
Kathleen Conery | Pharyngeal pumping behavior in C. elegans is employed to ingest bacteria, the worms’ normal food. Under laboratory conditions, C. elegans are reared on agar plates seeded with the E. coli strain OP50, which stimulates feeding behavior. Accordingly, we tested the ability of OP50 treatment to elicit pharyngeal pumping, using the ScreenChip system.
Kathleen Conery | The neuromodulator serotonin (5-hydroxytryptamine; 5HT) is often used to stimulate pharyngeal pumping in C. elegans and other nematodes. To investigate the concentration-dependence of 5HT treatment on pump frequency measured in the ScreenChip platform, we tested the effects of 0, 2, 5 and 10 mM 5HT on N2 (wild type) adults.
Kathleen Conery | Most existing anthelmintics act on ion channels or neurotransmitter receptors, so determining whether a candidate compound impairs electrical signaling is useful to know early in the screening process. Essential oil of fennel (Foeniculum vulgare) has been used traditionally in South America as a worming agent against human infections with the trematode parasite, Schistosoma mansoni. Using the ScreenChip platform, we investigated the effect of fennel essential oil as an anthelmintic on C. elegans.